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. Author manuscript; available in PMC: 2008 Oct 24.
Published in final edited form as: Brain Res. 2007 Aug 24;1176:71–81. doi: 10.1016/j.brainres.2007.08.036

Figure 4. Levels of uncoupling (UCP) proteins in mitochondria of female and male rat brains.

Figure 4

Bands migrating at 32 kDa were detected using antibodies directed against UCP-2, UCP-4, and UCP-5. Porin bands (31 kDa) are shown for the UCP-2 blot, and were run for each blot as a mitochondrial specific protein loading control. A. Representative Western blots show UCP-2, 4 and 5 protein in mitochondria isolated from intact, ovariectomized (OVX), and estrogen-treated OVX (OVX+E) female rat brains. B. Representative Western blots show UCP-2, 4 and 5 protein in mitochondria isolated from intact, orchiectomized (ORX), testosterone-treated ORX (ORX+T), DHT-treated ORX (ORX+DHT), and estrogen-treated ORX (ORX+E) male rat brains.