Figure 2.

Formation of single colonies on agar plates with or without arabinose. Competent E. coli N4156::pAra14-FtsY′ (13) was transformed with pCL1291 derivatives harboring the various ftsY gene constructs as indicated. The transformants were incubated in LB supplemented with 0.2% arabinose at 37°C. One hour later, the recovered transformants were centrifuged, washed once in LB without arabinose, and spread on LB plates containing ampicillin (100 μg/ml), spectinomycin (100 μg/ml), IPTG (0.1 mM), and with or without arabinose. Photographs were taken after incubation for 36 hr at 37°C.