Skip to main content
. 2007 Oct 2;4:71. doi: 10.1186/1742-4690-4-71

Figure 6.

Figure 6

Generation of a proviral DNA construct expressing the antisense directed luciferase gene expression. (A) 293T cells were transfected with 600 ng pNL4.3AsLucR-E- and lysed 48 h post-transfection. CTL represents untransfected cells. (B-C) Jurkat cells were transfected with 15 μg pNL4.3AsLucR-E- (B) or pNL4.3ΔBstAsLucR-E- (C) and stimulated with different activators for 8 h. or left untreated. Lysed cells were then analysed for luciferase activity. Results show the mean luciferase activity values of three measured samples ± S.D (A-B) or fold induction compared to the unstimulated control (C).