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TIN2 binds to SA1. (A–C) TIN2N not TIN2C is co-immunoprecipitated by SA1 or SA1Δ72, not SA2. 293T cells were transfected with GFPTIN2N or GFPTIN2C and (A) FlagSA1, (B) FlagSA1Δ72, or (C) FlagSA2. Cell lysates were immunoprecipitated with anti-Flag beads. Proteins were fractionated on SDS–PAGE and analyzed by immunoblotting with antibodies against Flag, GFP, or Smc3. Input indicates 4% of extract. (D) Schematic representation of the interactions between SA1 and TRF1 and TIN2.
