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. 2007 Nov 1;26(23):4856–4866. doi: 10.1038/sj.emboj.7601905

Figure 6.

Figure 6

The purified CITFA complex is transcriptionally active. (A) Immunoblot of mock-treated and PTP-CITFA-2-depleted (depl) transcription extract (Textract). PTP-CITFA-2 and TFIIB were co-detected with the PAP reagent and a polyclonal antibody, respectively. (B) Co-transcription of class I templates and SLins19 in mock-treated or CITFA-2-depleted extract. The depleted extract was complemented with final eluate of TFIIB (depl+TFIIB-P) or CITFA-2 (depl+P-CITFA-2) PTP purifications. Marker, pBR322-MspI.