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Whole-mount in situ hybridization for a zebrafish sequence orthologous to ngf60, showing its expression pattern in the brain 48 h past fertilization (hpf). For comparison, the expression pattern is also shown for OTP, a homeobox transcription factor that was used as a positive control because of its highly specific and well described expression profile (Eaton and Glasgow 2007). The expression patterns of the two genes remain generally similar at 72 hpf (Supplemental material).
