Table 1.

Chromatographic properties of N-acyl-HSLs

HSL	Rf*	Amount applied (in pmol) required
		For minimum detection	For routine detection†
3-Oxo-C4	0.82	NK	NK
3-Oxo-C6	0.68	0.03	0.52
3-Oxo-C8	0.41	0.0005	0.03
3-Oxo-C10	0.18	0.19	3
3-Oxo-C12	0.07	2	16
3-Unsubstituted C4	0.77	ND	ND
3-Unsubstituted C6	0.47	300	1200
3-Unsubstituted C8	0.23	2.4	38
3-Unsubstituted C10	0.09	100	400
3-Unsubstituted C12	0.02	NK	NK

NK, not known. Amounts available were insufficient to determine weights. ND, not detected. 

^*R_f values differ slightly from one experiment to the next. The values given represent migration rates taken from a single experiment in which all of the standards were chromatographed on the same plate. 

^†Values represent amounts of the indicated N-acyl-HSL required to produce an easily detectable reaction with the A. tumefaciens indicator strain (see Fig. 1).