FIG. 2.

H2A.Z is excluded from pericentric heterochromatin in differentiated mouse fibroblasts. (A) Mouse 10T1/2 fibroblasts were stained using antibodies against H2A.Z and HP1α and were examined by immunofluorescence microscopy. Both H2A.Z antibodies show punctate nuclear staining but are excluded from the DAPI-dense and HP1α-enriched pericentric heterochromatin. (B) Metaphase chromosome spreads from mouse 10T1/2 cells show the exclusion of H2A.Z from the pericentric heterochromatin. The mutually exclusive localization of H2A.Z and HP1α is seen both at the pericentric heterochromatin and on the chromosome arms.