FIG. 6.

Antibodies directed against the C terminus of H2A.Z do not recognize the monoubiquitylated form of H2A.Z. Total cell lysates from 293T cells were examined by Western blot analyses using the indicated H2A.Z antibodies. Both the H2A.Z-C antibody and the commercially available antibody have difficulty detecting the endogenous monoubiquitylated H2A.Z. The bands marked by an asterisk represent nonspecific proteins within the total cell extracts that are recognized by the Abcam H2A.Z antibody.