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. 2007 Jul 16;27(18):6520–6531. doi: 10.1128/MCB.00733-07

FIG. 5.

FIG. 5.

Cbp80p impedes utilization of the weak gal10-Δ56 terminator. (A) Schematic showing the GAL10-GAL1 gene pair with the Δ56 mutation in the GAL10 terminator. Arrows depict locations and relative abundances (proportional to line thickness) of transcripts produced in strains of the indicated genotypes and Gal phenotypes. RT, read-through. (B) cbp80Δ and npl3Δ partially suppress the Gal− phenotype of gal10-Δ56 cells. Tenfold serial dilutions of cbp80Δ and npl3Δ cells were spotted on yeast extract-peptone plates containing either 2% glucose (left) or 2% galactose (right) as carbon sources for 4 days. Rows 1 to 3, CMY127 (WT), CMY128 (cbp80Δ), and CMY129 (npl3Δ) strains with gal10-Δ56; rows 4 to 6, isogenic GAL10 strains, BY4741 (WT), 6566 (cbp80Δ), and 4268 (npl3Δ). (C) Northern analysis of GAL10, GAL7, and GAL10::GAL7 read-through mRNAs. Total RNAs (20 μg) from wild-type (GAL10+) strain BY4741 and gal10-Δ56 strains CMY127 (WT), CMY128 (cbp80Δ), and CMY129 (npl3Δ) induced with 2% galactose for 3 h were resolved and probed for GAL10 or GAL7 mRNAs and the Pol III transcript SCR1 (as a loading control). The read-through transcript that hybridizes with both GAL10 and GAL7 probes is marked (RT).