FIG. 5.

Analysis of CTSBP2 forms with pairwise subdivisions of penta-alanine mutations. The RFQDR^647-651 segment and the flanking segments subjected to penta-alanine mutation (LLKEL^641-645 and RRLVL^664-668) were each analyzed in four mutant proteins (indicated by the corresponding mutation) with pairwise substitutions of alanine. Each mutant protein harboring a pairwise substitution, along with the corresponding penta-alanine mutant protein (indicated by the region corresponding to the mutation), was assayed for ribosome binding, SECIS binding, and Sec incorporation activity. The data are the means (± standard errors) of results from at least three independent experiments.