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. 2007 Jul 9;27(18):6279–6287. doi: 10.1128/MCB.00978-07

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Copyright © 2007, American Society for Microbiology

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FIG. 5. — A chd1 mutation suppresses the synthetic growth defect of spt16 with set1 or histone H3(K4R) mutations. (A) A chd1 mutation suppresses the spt16 set1 synthetic growth defect. Tenfold dilutions of strains DY150 (wild type), DY8875 (set1), DY8107 (spt16), DY9206 (spt16 set1), and DY9271 (spt16 set1 chd1) were plated on complete medium at 30°C or 33°C for 2 days. (B) A chd1 mutation suppresses the spt16 histone H3(K4R) synthetic growth defect. Tenfold dilutions of strains DY8862 [hht1-hhf1 hht2-hhf2 + YCp-TRP1:H3(wild type)-H4(wild type)], DY8865 (spt16 hht1-hhf1 hht2-hhf2 + YCp-TRP1:H3(wild type)-H4(wild type)], DY8863 [hht1-hhf1 hht2-hhf2 + YCp-TRP1:H3(K4R)-H4(wild type)], DY8866 [spt16 hht1-hhf1 hht2-hhf2 + YCp-TRP1:H3(K4R)-H4(wild type)], and DY10472 [spt16 chd1 hht1-hhf1 hht2-hhf2 + YCp-TRP1:H3(K4R)-H4(wild type)] were plated on complete medium at the indicated temperature for 2 days.