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. 2007 Jul 9;27(18):6279–6287. doi: 10.1128/MCB.00978-07

FIG. 8.

FIG. 8.

A chd1 mutation suppresses the synthetic lethality of an spt16 mutation with either TBP or TFIIA mutations. (A) Strains DY8552 (spt15 spt16 + YCp-URA3-TBP-Spt16) (indicated as “CHD1”) and DY10141 (spt15 spt16 chd1 + YCp-URA3-TBP-Spt16) (indicated as “chd1”) were transformed with two plasmids, a YCp-TRP1 plasmid encoding a TBP mutant and a YCp-LEU2 plasmid with either wild-type SPT16 or spt16 mutations, and 10-fold dilutions were plated at 33°C either on complete medium for 2 days or on FOA medium for 3 days. (B) Strains DY8700 (spt16-11 toa2 + YCp-URA3-TOA2) (indicated as “CHD1”) and DY10214 (spt16-11 chd1 toa2 + YCp-URA3-TOA2) (indicated as “chd1”) were transformed with a YCp-LEU2 plasmid with the indicated toa2 mutant, and 10-fold dilutions were plated for 2 days on complete medium at 25°C and on FOA medium at 30°C.