Additive suppression of pob3 by chd1 and set2 mutations. (A) Tenfold dilutions of strains DY150 (wild type), DY9809 (chd1), DY8690 (set2), DY9838 (chd1 set2), DY7379 (pob3), DY9458 (pob3 chd1), DY8878 (pob3 set2), and DY9547 (pob3 chd1 set2) were plated on complete medium at 25°C or 32°C for 3 days. (B) Tenfold dilutions of strains DY150 (wild type), DY9809 (chd1), DY8690 (set2), DY9838 (chd1 set2), DY8107 (spt16), DY9152 (spt16 chd1), DY8777 (spt16 set2), and DY9153 (spt16 chd1 set2) were plated on complete medium at either 30°C or 35°C for 2 days. (C) Tenfold dilutions of strains DY8777 (spt16 set2), DY9153 (spt16 set2 chd1), DY11619 (spt16 set2 chd1 CHD1-HA), DY11626 [spt16 set2 chd1 CHD1(ΔCD)-HA], and DY11645 [spt16 set2 chd1 CHD1(K407R)-HA] were plated on complete medium at 30°C for 2 days or at 35°C for 3 days.