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ORF1p colocalizes with marker proteins of stress granules. Fluorescent-tagged ORF1p was coexpressed in 143Btk- or 293T cells with fluorescence-tagged control proteins (A and B), marker proteins for PBs (C to E), and marker proteins for SGs (F to J). (G) Cells were cotransfected with TIA1-RFP and ORF1p-GFP containing a double point mutation, N157A/R159G, that prevents formation of cytoplasmic foci. Cells were imaged by confocal microscopy at ×100 magnification. Bar, 10 μm.
