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. 2007 Jun 11;27(18):6469–6483. doi: 10.1128/MCB.00332-07

FIG. 5.

FIG. 5.

FIG. 5.

ORF1p colocalizes with other RNA-binding proteins in stress granules. (A to E) GFP-tagged ORF1p was coexpressed in 143Btk- or 293T cells with RFP-tagged proteins identified from the two-hybrid screen. Cells were treated with 600 mM sorbitol or left untreated. (F to L) Localization of endogenous, GFP-fused, or T7 epitope-tagged ORF1p with endogenous proteins in teratocarcinoma cells. Cells were left untreated or treated with thapsigargin or sorbitol 2 h prior to fixation. In panel L, yellow arrows show alignment of FMRP with ORF1 protein in SGs. White arrows show smaller foci formed by FMRP, presumably PBs, which do not align with ORF1p. (M) Coexpression of endogenous FMRP and TIA-1 proteins in treated and untreated teratocarcinoma cells. Cells were imaged with a Nikon E600 microscope at ×60 magnification or confocal microscopy at ×100 magnification. Bar, 10 μm.