Table 1.

N-terminal sequence of major peptides obtained after limited proteolysis of annexin A7 with trypsin or chymotrypsin.

		Trypsin			Chymotrypsin
	Site	N-terminal Sequence		Site	N-terminal Sequence
Peptide 1	U	G S ? Y P	(33 kDa)	Y110	– G – P A	(37 kDa)
Peptide 2	U	G S ? Y P	(28 kDa)	F160	D A – R D	(33 kDa)

The proteolysis products were separated by SDS-PAGE, transferred to PVDF membranes and stained with Coomassie blue. The relevant bands were excised and processed for N-terminal sequencing. The N-terminal sequence of recombinant annexin A7 was verified to be GSSYP. The potential sequences were GSSYP for both trypsin generated and GGGPA and DAMRD for the chymotrypsin generated 37 and 33 kDa peptides, respectively. U – unknown, but possibly in the COOH-terminus. The undetermined sequence is indicated by ? and – indicates a non-matching residue.