Figure 2.
Evaluation of the radiosensitivity of wild type and PP2Cα siRNA-expressing MCF7 cells. A: Effect of different doses of 60Cobalt γ-radiation on the proliferation of MCF7-wt and MCF7-si cells. Values represent average ± SD (n = 4). B: Effect of radiotherapy on the clonogenicity of MCF7-wt and MCF7-si cells. Values represent average ± SD (n = 4). C and D: Quantification of the effect of radiotherapy on the viability (i.e. the membrane integrity) of MCF7-wt and MCF7-si cells. The viability was determined by means of FACS analysis. Values represent average ± SD (n = 3). E: Representative images of the cell cycle distribution of MCF7-wt and MCF7-si cells at 48 h after treatment with radiotherapy.