Table 1.

Cell cycle analysis of wild type and PP2Cα siRNA-expressing MCF7 cells.

Time (hours post trypsination)	MCF7-wt			MCF7-si
	G0/G1	S	G2/M	G0/G1	S	G2/M
Control	54.5 ± 1.5	33.5 ± 0.4	12.0 ± 1.3	44.9 ± 2.4 *	32.4 ± 1.6	22.7 ± 3.7 *
6	74.0 ± 0.5	17.3 ± 0.2	8.6 ± 0.4	50.2 ± 2.8 *	34.4 ± 2.4 *	16.1 ± 4.2 *
12	74.1 ± 0.2	16.7 ± 0.8	9.2 ± 0.7	46.1 ± 3.6 *	33.5 ± 3.1 *	20.4 ± 6.7 *
24	48.0 ± 0.9	41.7 ± 0.4	10.3 ± 0.9	57.8 ± 2.7 *	23.8 ± 3.5 *	18.4 ± 1.1 *
48	48.4 ± 0.7	39.9 ± 0.4	11.6 ± 0.8	59.4 ± 4.8 *	25.1 ± 0.3 *	15.5 ± 4.9
72	51.9 ± 0.5	34.6 ± 0.5	13.5 ± 0.3	46.3 ± 2.7 *	31.3 ± 1.0 *	21.8 ± 2.5 *

Multicycle algorithm-based quantification of the amounts of MCF7-wt and MCF7-si cells in the G0/G1, the S and the G2/M phase of the cell cycle. Results are shown both for confluently growing control cells and for cells analyzed at various time points after trypsination and (re-) seeding. Values represent average ± SD (n = 3). * Indicates p < 0.05 vs. MCF-wt.