Table 3.

Cell cycle analysis of wild type and PP2Cα siRNA-expressing MCF7 cells upon treatment with chemotherapy.

		MCF7-wt			MCF7-si
Time (h)	Dox Dose (μM)	G0/G1	S	G2/M	G0/G1	S	G2/M
24	0	53.6 ± 0.5	33.3 ± 1.2	13.1 ± 0.3	56.6 ± 0.5 *	29.7 ± 0.6 *	13.7 ± 0.9
	1	74.8 ± 0.9	8.0 ± 0.6	17.2 ± 0.3	53.9 ± 0.6 *	30.7 ± 1.0 *	15.4 ± 1.3
	2.5	50.4 ± 0.7	4.1 ± 0.8	45.5 ± 1.5	52.1 ± 0.9	5.0 ± 2.7	42.8 ± 1.9
	5	45.1 ± 0.2	6.4 ± 0.2	48.5 ± 0.1	51.5 ± 1.6 *	5.1 ± 2.4	43.4 ± 0.9 *
	10	38.9 ± 1.3	29.5 ± 0.8	31.6 ± 0.8	41.3 ± 0.3 *	32.5 ± 1.6 *	26.2 ± 1.5 *
	20	40.8 ± 1.8	31.3 ± 2.5	27.8 ± 1.3	54.9 ± 1.4 *	30.2 ± 0.5	14.9 ± 1.4 *
48	0	61.5 ± 0.6	29.1 ± 1.0	9.4 ± 0.7	61.2 ± 1.8	29.5 ± 1.7	11.2 ± 0.1 *
	1	71.4 ± 3.5	8.9 ± 1.7	19.7 ± 5.2	52.2 ± 2.7 *	2.5 ± 1.2 *	45.3 ± 1.7 *
	2.5	35.7 ± 1.3	3.9 ± 0.3	60.4 ± 1.0	58.3 ± 5.8 *	3.2 ± 0.6	38.5 ± 5.3 *
	5	31.7 ± 0.5	5.2 ± 1.6	63.2 ± 1.1	49.6 ± 1.0 *	2.5 ± 0.7	47.8 ± 0.4 *
	10	30.3 ± 1.0	23.6 ± 0.8	46.1 ± 0.7	44.0 ± 1.7 *	8.4 ± 0.6 *	47.6 ± 2.3
	20	33.2 ± 1.8	48.6 ± 7.7	18.3± 9.4	50.3 ± 0.8 *	43.4 ± 2.4	6.4 ± 1.6
72	0	71.9 ± 1.7	22.8 ± 0.7	5.4 ± 1.3	59.2 ± 0.9 *	30.4 ± 1.6 *	10.5 ± 0.6 *
	1	52.5 ± 7.2	3.1 ± 1.9	44.4 ± 8.9	47.4 ± 7.3	0.5 ± 0.9	52.1 ± 8.2
	2.5	46.4 ± 1.2	0.5 ± 0.5	53.3 ± 2.0	41.3 ± 2.6 *	1.9 ± 3.0	56.8 ± 1.8
	5	38.8 ± 0.3	0.1 ± 0.1	61.3 ± 0.3	40.4 ± 0.8 *	1.8 ± 1.6	57.8 ± 1.5 *
	10	34.1 ± 0.7	16.4 ± 1.7	49.5 ± 2.3	37.5 ± 1.5 *	3.5 ± 2.5 *	57.1 ± 2.6 *
	20	32.8 ± 2.8	39.9 ± 1.8	27.3 ± 1.8	N.Q.	N.Q.	N.Q.

Multicycle algorithm-based quantification of the amounts of MCF7-wt and MCF7-si cells in the G0/G1, the S and the G2/M phase of the cell cycle at 24, 48 and 72 h after treatment with the indicated doses of doxorubicin. Values represent average ± SD (n = 3). N.Q. (Not Quantifiable) indicates that multicycle algorithm failed to attribute the cells to a certain cell cycle phase. * Indicates p < 0.05 vs. MCF-wt.