Fig. 2.

Confocal laser imaging of GFP-positive mesenchymal stem cells in suspension. All cultured mesenchymal stem cells show strong green autofluorescence. MSCs are also labelled with propidium iodide (red), a nuclear marker (A). Light micrographs of mesenchymal stem cells in suspension stained with haematoxylin and eosin (B–D). The cells have an eccentric nucleus and the cytoplasm is divided into two differently stained areas: a more intensely stained inner zone and a thin peripheral zone with a pale appearance. The plasma membrane showed an irregular profile. GFP-positive adherent cells (E,F) show a central, round nucleus and an irregular cytoplasm profile due to the presence of adherence pseudopodia. Scale bars, 10 µm.