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(a) Exogenous GDNF causes the tips of ureteric buds, shown here by staining for laminin, to expand. The unusually large tips remain free of DBA staining (b). Treatment of the Wolffian duct with exogenous GDNF causes the emergence of supernumerary ureteric buds (c); these too lack DBA staining at their tips (d). Scale bar = 100 µm.
