Fig 4.

External cranial morphology and skeletal development are indistinguishable among wild-type frogs (A,D), ROSA26:GFP transgenic frogs (C,F) and chimeric frogs (B,E). All animals were reared to at least 6 months of age; skeletal anatomy was assessed in cleared-and-stained whole-mounts (D–F). The three groups were indistinguishable from one another in terms of normal development and growth. The minor differences in cranial size among the three cleared-and-stained specimens depicted here are common even among populations of normal tadpoles reared under identical conditions but in different tanks. Scale bar, 2 mm.