Figure 2. Intracellular cytokine production by CD4 and CD8 T cells following immunization of BALB/c mice with Lutzomyia intermedia SGS.

BALB/c mice (3–5 per group) received 3 inoculations of PBS (open bars) or SGS (closed bars). Fifteen days after the last inoculation, draining lymph nodes were pooled and cells were preincubated with Brefeldin A for four hours before being stained. Data represent the percentages of cells with signals for the particular cytokine that were greater than the background signals established using isotype controls. Bars represent the means and standard errors of the means of one experiment, representative of three independent experiments.