Figure 5. Parasite load estimate in BALB/c mice immunized with L. intermedia SGS or PBS and challenged with L. braziliensis in the presence of L. intermedia SGS.
BALB/c mice (3–5 per group) were inoculated three times with PBS (open bars) or SGS (closed bars). Two weeks after the last inoculation, mice were challenged with 105 L. braziliensis+SGS (equivalent to 1 pair of salivary glands/animal). Ear (A) and draining lymph node (B) parasite loads were determined at two, four and 16 weeks post infection via a limiting dilution assay. Bars represent the means and standard errors of the means from three independent experiments. (*p<0.05)