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. Author manuscript; available in PMC: 2008 Nov 25.
Published in final edited form as: Virology. 2007 Aug 13;368(2):262–272. doi: 10.1016/j.virol.2007.07.009

Figure 5. Activation of IL-6 was independent of IFN-β.

Figure 5

(A) Activation of IL-6 expression in the U3A cells. The U3A cells were infected with mock, PIV5 or rPIV5VΔC at 5 MOI as before. Media were collected from infected cells at time points indicated. Amount of IL-6 in the media were measured using ELISA. (B) Effect of anti-IFN-β on the activation of IL-6. HeLa cells were infected and treated with 1 μg/ml of anti-IFN-β antibody or equal amount of control antibody. The relative levels of IL-6 mRNA were measured using qRT-PCR. (C) Effect of IRF-3DN on the activation of IL-6. HeLa cells were transfected with plasmids encoding V, VΔC, P or IRF-3DN and p1168hIL-6-F-Luc and phTK-RL. The transfected cells were then infected with, mock, PIV5 or rPIV5VΔC. Dual luciferase assays were carried out as described in Materials and Methods. The relative luciferase activity indicates ratio of F-Luc to R-Luc. Error bars are standard deviation of mean (SEM).