Figure 5.

Synthetic protein-free oligonucleotides as targets of junction endonuclease. The synthetic substrates at 2.5 μM were incubated with 1 ng/μl of purified fraction 9 junction endonuclease (JE) at 37°C for the times indicated. Digestion with P1 nuclease at 0.125 × 10⁻³ units/ml was used to identify the single-stranded region. The reaction was stopped by adding 50% formamide and 20 mM EDTA. The samples were loaded onto a 12% denaturing polyacrylamide gel. (A) The eye substrate. (B) The bubble substrate (see Fig. 1B).