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Cyclopamine treatment leads to downregulation of Shh targets in the forelimb. Within two hours, Ptc1 is downregulated in treated forelimbs (B) when compared to control forelimbs (A), and its expression ceases within four hours of cyclopamine treatment (C). Gli1 and Bmp2 are downregulated within eight hours (D,F) compared to control forelimbs (E,G). On the other hand, Gli2 expression expands into the posterior forelimb within eight hours of treatment (H) when compared to controls (I).
