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. Author manuscript; available in PMC: 2008 Sep 15.
Published in final edited form as: Dev Biol. 2007 Jul 12;309(2):373–385. doi: 10.1016/j.ydbio.2007.07.004

Figure 1. Zebrafish lmo4b is essential for viability and eye, ear and pectoral fin development.

Figure 1

(A) Alignment of amino acid sequence alignment of zebrafish (Dr.) Lmo4b (Acc. #NP817093) with mouse (Mm.; Acc. #NM010723.1), human (Hs.; Acc. #NM006769.2) and zebrafish (Dr.) Lmo4a (Acc. #AAN03596). Residues unique to Lmo4b are underlined in red. (B) Western blot of extracts from embryos injected with 500pg of 6xmyc-lmo4b mRNA and a mixture of two non-overlapping translational-blocking MOs or a 4mm control MO, probed with anti-myc antibody. An unrelated immunoreactive band present in all extracts, demonstrates comparable loading for all lanes. (C–J) 72 hpf larvae or larval tissue from embryos injected with 4ng of either 4mm-MO (C, E, G, I) or 4ng of lmo4b-MO (D, F, H, J). High magnification views of morphant eyes reveal retinal enlargement (F) and defects in the ear (G, H) and pectoral fins (I, J).