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. 1997 Jun 10;94(12):6087–6092. doi: 10.1073/pnas.94.12.6087

Figure 6.

Figure 6

Reconstitution of the [4Fe-4S] cluster in FNR from the [2Fe-2S] cluster. (A) Absorption spectrum of air-oxidized FNR (thick line) and the same after reconstitution of the [4Fe-4S] cluster with Azotobacter vinelandii NifS protein as described (thin line). The reference cuvette contained the same mixture except for FNR. (B) DNA binding of air-oxidized (10 min) FNR lacking the 4Fe cluster (1), air-oxidized FNR following reconstitution of the [4Fe-4S] cluster (2), and FNR as obtained on purification (3).