Figure 4.

Transient expression of the GlcAT-P cDNA in Lec2 cells. Mock transfected and transfected cells were incubated with normal mouse IgM (A and E), H11 (anti-paragloboside antibody, IgM) (B and F), M6749 (IgM) (C and G), and HNK-1 (IgM) (D and H), followed by fluorescein isothiocyanate-conjugated anti-mouse IgM. About 50% of the cells transfected with a GlcAT-P cDNA carried in mammalian expression vector pEF-BOS were stained with M6749 (G), but not at all with HNK-1 (H). No immunoreactivity was detected in mock-transfected cells (C and D). The immunoreactivity of Lec2 cells to anti-paragloboside antibody was not changed by transfection with GlcAT-P cDNA (B and F).