Figure 5.
Transient expression of the GlcAT-P cDNA in COS-1 cells. COS-1 cells were transfected with the GlcAT-P cDNA in pEF-BOS (A and B) and with vector alone (C) as described in Materials and Methods. Indirect immunofluorescence staining of HNK-1 epitope (A) and corresponding phase-contrast micrograph (B). The GlcAT-P cDNA transfected cells were heavily stained with HNK-1, but mock transfected cells (C) were not. HNK-1 positive cells extended long processes with a number of microspikes. (Bar, 50 μm.)