Figure 2.

Identification of HL-60 chrysin-binding proteins p44, p40, and p29 as the individual subunits of CKII. (A) Flow chart for chemical analyses. After SDS/PAGE and electroblotting onto poly(vinylidene difluoride), p44, p40, and p29 were excised and subjected to the indicated analyses. (B) Silver stain of chrysin-binding proteins in lysates pretreated with 10 μM chrysin or 10 μM DRB, or in a control lysate using alkaline phosphatase to digest binding proteins just before electrophoresis. (C) Immunoblot analysis of replicate samples shown in B, using a mixture of two polyclonal antibodies to enable the viewing of all three CKII subunits. One antibody recognizes both the α and α′ chains of CKII, and the second is reactive toward the β chain.