Figure 4.

Inhibition of HIV-1 expression in OM-10.1 cells by nontoxic concentrations of DRB. Immediately before induction of HIV-1 expression by 20 units/ml TNF-α, cultures received specified concentrations of DRB. Forty-eight hours later, reverse-transcriptase activity (▵) was measured in culture supernatants, cell viability (○) was assessed by flow cytometric analysis of propidium iodide exclusion, and cell number (□) was measured via reduction of the formazan dye MTS. All values are expressed as a percent of the result from TNF-α treatment alone.