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Activation of adenylyl cyclase. αs was purified from rabbit liver membranes (•) or after expression in Sf9 cells (▴) or E. coli (▪). The proteins were activated with GTP[γS] and reconstituted with membranes (5 μg) from Sf9 cells expressing type V adenylyl cyclase. Adenylyl cyclase activity was assayed as described. Values shown are the average of triplicate determinations and are representative of at least two experiments.
