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Rate of reaction of AFB1 exo-8,9-epoxide with DNA measured by fluorescence changes. (Inset) Rate of fluorescence decrease upon reaction in the presence of DNA (0.10 mg⋅ml−1 = 0.32 mM monomer equivalents). The observed pseudo-first order single exponential rate is 1.9 s−1. These data were fit to a quadratic equation for a hyperbola represented by the line.
