Table 1.
Low affinity form | High affinity form | |
---|---|---|
Selenocysteine in C-terminal | ||
tryptic peptide | + | + |
Subunit mass | ||
by SDS/PAGE | 57 kDa | 57 kDa |
NADPH linked | + | + |
Specific activity (DTNB) | 26 units/mg | 32 units/mg |
Bound cofactor | FAD | FAD |
Heparin binding | − | + |
Reaction with rat liver | ||
polyclonal antibody | + | − |