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. 1997 Jun 10;94(12):6142–6147. doi: 10.1073/pnas.94.12.6142

Figure 2.

Figure 2

The Cdc18-associated kinase is a cdk containing Cdc2. (A) Purified GST, GSTcdc18, and GSTorp2 were separated on an SDS/polyacrylamide gel, transferred to nitrocellulose, and probed with antibodies to GST (Left), Cdc2 (Center), and the PSTAIRE region of Cdc2 (Right). Cdc2 translated in vitro (IVT Cdc2) was included in the anti-PSTAIRE immunoblot as a marker for Cdc2. (B) Purified GSTcdc18 was assayed for H1 and GSTcdc18 kinase activity in the presence of GST (−) or GSTrum1 (+). (C) GSTcdc18 was affinity-purified from cdc2+, cdc2–33, and cdc2-L7 strains, and the associated kinase was assayed using histone H1 as a substrate at 25, 37, and 40°C.