Final concentration	Amount of stock solution to make 50 ml
50% formamide	25 ml formamide
5X SSC	12.5ml of 20 X SSC (see below)
0.3 mg/ml yeast RNA (ICN)	0.3 ml of 50 mg/ml stock in DEPC-treated water
100 μg/ml heparin (Sigma)	100 μl of 50 mg/ml stock in DEPC-treated water
1X Denhardt's Solution	0.5 ml of 100X stock (see below)
0.1% Tween 20	0.5 ml of 10% Tween 20 in DEPC-treated water
0.1% CHAPS	0.5 ml of 10% CHAPS in DEPC-treated water
5 mM EDTA	0.5 ml of 0.5 M EDTA (pH 8.0) in DEPC-treated water
3 μl/ml DNA random primer	150 μl of stock (see below)