Figure 6.

Expression pattern of CML33 transcript in human myeloid leukemia cells HL-60 induced to undergo monocytic differentiation after PMA treatment for various intervals. Total RNA (10 μg) isolated from untreated exponentially growing cells (0) and cells treated for 4, 16, 24, 36, and 96 h were analyzed on Northern blots with the CML33 cDNA probe. Note marked reduction in the level of transcript with induced differentiation after prolonged treatment with PMA. The same blot was hybridized with a control β₂-microglobulin (β MG) probe to demonstrate comparable amount of RNA loading in each lane.