Figure 3.

NSF-driven disassembly renders syntaxin, SNAP-25, and synaptobrevin susceptible to cleavage by the light chains of BoNT/C1, BoNT/A, and TeNT, respectively. Disassembly was carried out in parallel in separate incubation that included, in addition to the toxin-free controls, BoNT/C1 L chain, BoNT/A L chain, and TeNT L chain, respectively, each at 2 μM. All samples were heated to 100°C before electrophoresis. Control incubations contained 10 mM EDTA. See Fig. 1 for antibodies and detection procedures.