Figure 4.

TeNT enhances NSF-driven disassemby by preventing reassembly during the disassembly reaction. Experimental conditions are identical to those described in Fig. 2 except that only an antibody specific for syntaxin was used and that immunoreactive bands were detected by a ¹²⁵I-Protein A procedure for quantitation (26). In b, each band was quantitated by PhosphorImaging (Molecular Dynamics). Shown is the average relative intensity for each of the experimental conditions. Toxin and control incubations were carried out as described in Fig. 3.