Figure 5.

Developmental morphology of cotB::rd28 strain and the effect of 100 mM sorbitol on this process. Cells were allowed to aggregate and form fruiting bodies synchronously on filters (PDF medium). (H) A typical fruiting body of the control line (AX4) after 24 hr of development. (A–F) Aggregates and fruiting bodies of the cotB::rd28 cells after 20 hr (A), 24 hr (B and C), and 40 hr (D–F). (G) The results shown are similar to those shown in D–F, but 100 mM sorbitol has been added to the PDF medium. This addition ameliorates the development of fruiting bodies in the RD28-expressing cells.