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. 1997 Jun 10;94(12):6202–6209. doi: 10.1073/pnas.94.12.6202

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Copyright © 1997, The National Academy of Sciences of the USA

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Whole mount in situ hybridization of rd28, cotB, and ecmA mRNA in cellular aggregates. AX4 and cotB::rd28 cells were allowed to develop for 16 hr (AX4 and cotB::rd28), fixed, permeabilized, and probed with antisense mRNA for rd28 (A and D), cotB (B and E), or ecmA (C and F). rd28 is not expressed in AX4 cells (D) and is only expressed in prespore cells in the transformed line (A); cotB is only expressed in prespore cells (B and E); and ecmA is only expressed in prestalk cells (C and F).