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Expression of exogenous GITR (A) and antisense GITR (RTIG) (B) in transfected 3DO cells as evaluated by competitive RT-PCR. The expected length of the PCR products was 1074 bp for GITR and RTIG and 851 bp for the competitor. (C) Anti-CD3-induced apoptosis of GITR or RTIG overexpressing clones in comparison to apoptosis of untransfected (nuc) and pCR3-transfected clones. The expression of TCR–CD3 was similar in transfected and untransfected clones (data not shown).
