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RETL1 and RETL2 can mediate GDNF dependent phosphorylation of RET. NB41A3 cells were transfected with the indicated plasmid and 48 hrs later treated with or without GDNF. Cells were lysed, precipitated with a mAb to RET, and subjected to Western blot analysis with either a polyclonal antibody to RET (Lower) or a mAb against phosphotyrosine (Upper).
