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Expression of RETL1 and RETL2 mRNA in rat tissues. Northern blots (17) contained 4 μg per lane of poly(A)+ mRNA from the indicated tissue. RETL1 and RETL2 cDNA probes of 1.3 kb and 1.4 kb, respectively, were generated by random priming (18). Human β-Actin and glyceraldehyde-3-phosphate dehydrogenase (G3PDH) control oligonucleotide probes (CLONTECH) were 5′ end-labeled with 32P using T4 polynucleotide kinase.
