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. 1997 Jun 10;94(12):6244–6249. doi: 10.1073/pnas.94.12.6244

Table 2.

Distribution of PNGase and marker enzyme activities on the discontinuous sucrose gradient centrifugation

Fraction PNGase
Lactate dehydrogenase
Glucose 6-phosphatase
UDP-Gal:galactosyl transferase
mU mU/mg Unit Unit/mg Unit Unit/mg mU mU/g
2 ND ND ND ND 0.031 0.14 0.00611 27
5 0.385 4.8 ND ND 0.045 0.56 0.00319 40
8 0.156 0.87 ND ND 0.08 0.45 0.00429 24
15 ND ND ND ND 0.095 0.24 0.0121 30
20 3.28 12.9 1.01 4.0 0.0092 0.036 0.00150 5.9

ND, not detected; mU, milliunits.