Table 3.

Summary of purification of hen oviduct PNGase

Fraction	Total activity,* mU	Amount of protein,† mg	Specific activity, mU/mg	Yield, %	Purification, -fold
Homogenate	1,910	19,700	0.0971	100	1.0
Supernatant	3,860	10,000	0.386	200	4.0
1st butyl-Toyopearl	2,380	437	5.45	120	56
2nd butyl-Toyopearl	646	34.2	19.8	34	190
Sephacryl S-300	339	3.44	98.5	18	1,000

mU, milliunit. 

^*One unit of enzyme was defined as the amount of enzyme releasing 1 μmol of ¹⁴C-labeled peptide from asialo-[¹⁴C]fetGP I under assay conditions upon incubation at 25°C for 24 h. 

^†Protein amount was determined by the modified Lowry method (bicinchoninic acid) using BSA as a standard.