Figure 3.

Proximal elements required for neuron-specific enhancer activity. (A) The 1,116-bp neuron-specific enhancer of the zebrafish GATA-2 promoter was ligated to P5–GM2 and P6–GM2 (Fig. 1). It was also ligated to Xs–GM2 in which the GM2 gene is fused to the Xenopus elongation factor 1α gene’s minimal promoter (shown as a solid bar). (B) The bar graph shows the percentage of embryos microinjected with each construct that expressed GFP in the CNS. The number of microinjected embryos observed by fluorescence microscopy for each construct is shown in parentheses.